Fast Ion™ Plasmid Midi/Maxi Kit

Process: Gravity flow ion exchange resin

Sample Source: Plasmid DNA from bacteria

Max sample size: Midi 150ml/Maxi 400ml

Yields: Midi 200µg/Maxi 500µg

Applications
Plasmid DNA preparation
Transfection (non-endotoxin sensitive)
Sequencing
In Vitro Transcription
Microinjection

Description

The Fast Ion™ Plasmid Midi/Maxi Kits use pre-packed anion exchange resin columns to purify plasmid DNA from bacterial cultures. A modified alkaline lysis method and RNase treatment are used to generate cleared cell lysates with minimal genomic DNA and RNA contaminantion. Following binding of the plasmid DNA to the column, the contaminants can be washed off with wash buffer. Finally, the purified plasmid DNA is eluted with a high salt buffer and precipitated with isopropanol for desalting. The entire procedure can be completed in 120 minutes resulting in high purity plasmid DNA.
 

Quality Control: The quality of Plasmid Midi/Maxi Kit is tested on a lot-to-lot basis. The kits are tested by isolation of plasmid DNA from 100ml cultures of E.Coli DH5α containing the plasmid pBluescript (A600>2 units/ml). More than 400µg of plasmid DNA was quantified with a spectrophotometer. 1µg of the purified plasmid was used for restriction enzyme digestion with EcoRI and DNA is checked by agarose gel analysis.

Product Information
 

FYPM10
10 Maxi preps/kit
110ml PM1 Buffer
110ml PM2 Buffer
110ml PM3 Buffer
110ml PEQ Buffer
360ml PW Buffer
110ml PEL Buffer
200µl RNase A (50 mg/ml)
10 PM Columns
 

FYPI25
25 Midi preps/kit
110ml PM1 Buffer
110ml PM2 Buffer
110ml PM3 Buffer
110ml PEQ Buffer
360ml PW Buffer
220ml PEL Buffer
200µl RNase A (50 mg/ml)
25 PI Columns