Protein microarrays/FAST® Slides

Features and Benefits

• Glass slides coated with a proprietary nitrocellulose polymer

• Polymer binds proteins in a noncovalent, irreversible manner and can be probed using the same methods as traditional blots

• Microarrays may be used with chemiluminescent or radiographic detection systems

• Standard 25 x 76mm size is compatible with microarray scanners and robots

• Matrix retains proteins in near-quantitative fashion, producing antibody arrays with sensitivity down to antigen concentrations of 1pg/mL

• Coating provides excellent long-term stability of printed proteins

• Slides are suitable for many types of protein microarrays, including antibody arrays and microarray Westerns

• Superior protein binding capacity

• Highest sensitivity and dynamic range

• Excellent long-term stability of printed proteins

• Compatible with all detection methodologies

• Compatible with commercially available arraying robots

FAST Slides are suitable for many types of protein microarrays including antibody arrays and microarray Westerns. There are tremendous advantages to using FAST Slides over traditional ELISAs and Westerns including less sample required, better sensitivity, linearity and quantitation. The largest advantage is hundreds or thousands of antibodies or samples can be screened simultaneously.

 FAST® PAK

Protein Array Kits

FAST PAK protein array kits provide the necessary components for researchers to conveniently build and process protein microarrays.

FAST PAK kits are available for all FAST Slide formats (1-, 2, 8-, and 16-pads) and include protein arraying buffer, protein array blocking buffer, protein array wash buffer and incubation chambers.

Features and Benefits

• 1, 2, 8 or 16 array pads on each slide

• Each pad can be processed separately to increase the number of arrays on each slide and reduce sample volume

• Ideal for multiplex experiments, side-by-side comparisons, and control experiments all on the same slide

• Reproducible results from slide-to-slide and pad-to-pad

• Increased protein stability and enhanced signal intensity with FAST PAK array buffer

• Proprietary protein array blocking buffer ensures optimum signal-to-noise ratios and promotes specific binding

• Flexibility for detection by fluorescent, chemiluminescent, colorimetric, and radiographic methods

Applications

• ELISA format (sandwich assay) experiments using antibody arrays

• Reverse phase (micro-Western) arrays using cell or tissue lysates

• Purified protein arrays

• Carbohydrate arrays

• Lipids and other materials which can be arrayed on nitrocellulose

 FAST® Accessories

The Chip Clip™ slide holder securely holds one FAST® slide and incubation chamber, the slide and incubation chamber are easily inserted into and removed.  Side rails hold the incubation chamber firmly against the slide surface and is compatible with all 25 x 76mm (1" x 3") slides.

The FAST® Frame multi-slide plate is designed to hold four FAST® slides and the corresponding well incubation chambers, in a microplate footprint dimensions meeting Society for Biomolecular Screening standards.  Together with the 96-well spacing (9mm centre to centre) of the array pads on the FAST® slide this enables high-throughput processing of the microarrays and makes the loaded FAST® Frame compatible with automated liquid handling systems and 8-channel manual pipettors.  Up to 64 arrays can be processed simultaneously when using four 16 pad FAST® slides in one FAST® Frame.