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 Total Protein Extraction Kit

 
protein gel runs isolated from 8 different human tissues by the Total Protein Extraction Kit

Total protein was isolated from 8 different human tissues by the Total Protein Extraction Kit.  The gel shows 50µg total protein run on 4-20% gradient SDA-PAGE gel. Lane 1, Liver; lane 2, Kidney; lane 3, Brain; lane 4, Skeletal Muscle; lane 5, Spleen; lane 6, Lung; lane 7, Placenta; and lane 8, Stomach.

     Total Protein Extraction Kit Manual

     Total Protein Extraction Kit Certificate of Analysis

     Total Protein Extraction Kit Certificate of Analysis 2
 

Biochain's total protein extraction Kit is an excellent tool for initial purification and preparation of proteins from tissue and cells.

Total native protein isolated with this kit can be used for several downstream applications.

The kit is easy to use: no scraping, nofreeze-thaw cycles, and no sonication.  The Total Protein Extraction Kit is composed of several buffers and can be used for isolation of protein from 5 grams of tissue or cultured cells.

Isolated protein is ideal for Western Blotting, SDS-PAGE, Immunoprecipitation, Gel mobility shift, and many other protein assays.

  • Isolate protein from large samples

  • Isolate protein from tissues or cultured cells

  • Protein isolated is intact, and can be >250 kDa


Storage conditions
Store TM buffer at 4¡C and 50x PI buffer at -20¡C

 
 

 CNMCS Compartmental Protein Extraction Kit

 
The CNMCS Compartmental Protein Extraction Kit provides an innovative, easy-to-use and cost-effective method to sequentially isolate Cytoplasmic, Nuclear, Membrane, and Cytoskeleton proteins from tissues and cells. This unique kit contains a ready-to-use protease inhibitor cocktail to prevent protein degradation while isolating the 4 compartmental proteins. Superior separation of subcellular proteome fractions are easily demonstrated by clearly distinct protein patterns and precise subcellular localisations of marker proteins on gels. The CMCS kit is an excellent tool for the initial purification and preparation of proteins for downstream applications including SDS-PAGE, Western blotting, gel mobility shift, protein assays and many other procedures. One kit consists of five reagents enough to isolate four compartmental proteins from 5 grams tissues or or about 250 million cells.
 

Benefits

  • Convenient - all reagents included for the preparation of 4 cellular compartmental
    proteins

  • Fast - isolate four compartmental proteins in less than three hours

  • Reliable - superior-quality and highly reproducible separation
    CNMCS Kit components

  • Pure - minimal cross contamination

  • Simple - easy to use compared with other methods, such as differential centrifugations

Applications

  • Detection of differential post-translational modifications or differential subcellular localisation of target proteins

  • Enrichment of low-abundance proteins for visualisation and subsequent analysis

  • Preparation of nuclear extract from tissues and cultured cells

  • Preparation of cytoplasmic fractions for detection of protein abundance

  • Preparation of membrane and cytoskeleton fractions

     CNMCS Compartmental Protein Extraction Kit Certificate of Analysis
 

 
 

 CNM Compartmental Protein Extraction Kit

 

 

CNM compartmental protein extraction kit
 

 

Storage and Stability
Buffer C, W,N, M, and CS are stored at 2-8¡C. The 50 x PI solution should be stored at -20¡C. If the kit is going to be used for multiple extractions, aliquot the PI solution properly before storing. The kit is stable for 1 year when handled properly.

     CNM Compartmental Protein Extraction Kit MSDS

     CNM Compartmental Protein Extraction Kit 2 MSDS

     Protein Extraction Kit Manual

CNM Compartmental protein Extraction Kit has the same features as CNMCS Kit except that it excludes isolation of cytoskeletal proteins.  This unique kit can sequentially isolate 3 compartmental proteins, including cytoplasmic, nuclear, and membrane proteins. Reagents are sufficient for extraction of compartmental proteins from 5g tissue.

Example of CNMCS Protein Extraction from Mammalian Tissue

Total protein and compartmental proteins extracted from rat colon tissue using Biochain Total Protein Extraction Kit and CNMCS Compartmental Protein Extraction Kit were submitted to SDS-PAGE in 5 identical gels. Coomassie staining of one piece of gel indicated distinct protein pattern of respective fractions.  Immunoblotting of PVDF membranes transferred from four other pieces of gel against cytoplasmic marker protein for multiple extractions, aliquot the PI solution properly before GAPDH, nuclear marker protein Histone H1, membrane marker protein EGFR, and cytoskeleton marker protein Vimentin assigned the majorities of the marker proteins to their expected compartmental fractions. Lane 1: Total Protein; Lane 2: Cytoplasmic Protein; Lane 3: Nuclear Protein; Lane 4: Membrane Protein; Lane 5: Cytoskeleton Protein


 

Order CodeDescription

Size

Price

FK3013010CNMCS Compartmental Protein Extraction Kit up to 5g tissue

Each

£380.90

FK3012010CNMCS Compartmental Protein Extraction Kit up to 5g tissue

Each

£330.10

FK30130101CNMCS Compartmental Protein Extraction Kit: Buffer C 12ml

12ml

£65.19

FK30130105CNMCS Compartmental Protein Extraction Kit: Buffer CS 3ml

3ml

£65.19

FK30130104CNMCS Compartmental Protein Extraction Kit: Buffer M 6ml

6ml

£65.19

FK30130103CNMCS Compartmental Protein Extraction Kit: Buffer N 6ml

6ml

£65.19

FK30130102CNMCS Compartmental Protein Extraction Kit: Buffer W 25ml

25ml

£65.19

FK30110102Total Protein Extraction Kit: 50X PI 260ul

260ul

£247.70

FK30110101Total Protein Extraction Kit: TM Buffer 13ml

13ml

£117.33

FK3011010Total Protein Extraction Kit: up to 5g tissue

Each

£296.20

 

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