
Total protein was isolated from 8 different human tissues by the Total
Protein Extraction Kit. The gel shows 50µg total protein run on
4-20% gradient SDA-PAGE gel. Lane 1, Liver; lane 2, Kidney; lane 3,
Brain; lane 4, Skeletal Muscle; lane 5, Spleen; lane 6, Lung; lane 7,
Placenta; and lane 8, Stomach.
Total Protein Extraction Kit
Manual
Total Protein Extraction Kit
Certificate of Analysis
Total Protein Extraction Kit
Certificate of Analysis 2
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Biochain's total protein extraction Kit is an
excellent tool for initial purification and preparation of proteins
from tissue and cells. Total native protein isolated
with this kit can be used for several downstream applications.
The kit is easy to use: no scraping, nofreeze-thaw cycles, and no
sonication. The Total Protein Extraction Kit is composed of
several buffers and can be used for isolation of protein from 5 grams
of tissue or cultured cells. Isolated protein is ideal
for Western Blotting, SDS-PAGE, Immunoprecipitation, Gel mobility
shift, and many other protein assays.
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Isolate protein from large samples
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Isolate protein from tissues or cultured cells
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Protein isolated is intact, and can be >250 kDa
Storage conditions
Store TM buffer at 4¡C and 50x PI buffer at -20¡C
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