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Description
BioChain’s LDH Cytotoxicity Assay kit is a colorimetric assay that
quantitatively measures lactate dehydrogenase (LDH), a stable
cytosolic enzyme that is released into the cell culture supernatant
upon damage of the cytoplasmic membrane. Released LDH in culture
supernatants is measured with a 20-minute coupled enzymatic reaction:
LDH oxidizes lactate to pyruvate which then reacts with tetrazolium
salt INT to form formazan. The increase in the amount of formazan
produced in culture supernatant directly correlates to the increase in
the number of lysed cells. The formazan dye is water-soluble and can
be measured by 490 nm absorbance using a standard ELISA plate reader.
The LDH-cytotoxicity assay is fast, sensitive, convenient, and
precise, and is applicable to a variety of cytotoxicity studies.
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Easy and Simple – add solutions to the cells,
incubate and read the absorbance.
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Fast – short reaction time, no centrifuge
needed
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Accurate – absorbance strongly correlates to
number of lysed cells
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Sensitive – more sensitive than alkaline and
acid phosphatase-based assays
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Reliable – superior-quality and highly
reproducible data
Applications
Storage and stability
Store at -20ºC. The kit is stable for one year when handled properly.
LDH Cytotoxicity Assay Kit Manual
LDH Cytotoxicity Assay Kit MSDS
LDH Cytotoxicity Assay Kit
Certificate of Analysis
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