QBlueTM Cell Viability Assay Kit

Description

This homogeneous assay involves simply adding a single reagent, the QBlue reagent to the cell culture and measuring the fluorescence intensity (excitation wavelength = 530 - 570nm, emission wavelength = 590 - 620nm) after an incubation step. The QBlue reagent, like other resazurin-based assays, utilises the redox dye resazurin, which is not fluorescent, but upon reduction by metabolically active cells is converted into a highly fluorescent product (resorufin). Living cells can readily reduce this non-toxic reagent and the resulting increase in fluorescence intensity can be conveniently monitored using a fluorescence spectrophotometer or plate reader. Non-viable cells have no metabolic capacity and, thus, will not reduce the dye. Therefore, the fluorescence intensity observed in this assay is a true measure of the viable cells. The Qblue reagent has been optimised for maximum sensitivity, reproducibility and long shelf life. The reagent is compatible with all culture media and with all liquid handling systems for high-throughput screening applications in 96-well and 384-well plates.

• Safe – non-toxic

• Sensitive and accurate – as low as 100 cells can be accurately quantified

• High-Throughput – allows simultaneous processing of tens of thousands of samples per day

• Homogeneous and convenient – a single reagent-mix, incubate and measure

Applications

• Cell Proliferation assays - effects of cytokines, growth factor and nutrients

• Cytotoxicity and Apoptosis assays - evaluation of toxic compounds, anticancer antibodies and toxins

• Drug Discovery - high-throughput screening for anti-cancer drugs

Storage conditions

The QBlue reagent is light sensitive and should be stored in the provided amber bottle at 4°C. The Control Reagent is stable at -20°C.
Shelf life: 12 months

    QBlue Cell Viability Assay Kit Manual

    QBlue Cell Viability Assay Kit MSDS

    QBlue Cell Viability Assay Kit Certificate of Analysis
 

 QMTT Cell Viability Assay Kit

Description

The QMTT assay kit provides a convenient, sensitive, quantitative and reliable assay for determining the number of viable cells in a given culture. This homogeneous colourimetric assay is based on the conversion of a tetrazolium salt MTT, a pale yellow substrate, to formazan, a purple dye. The intensity of the product color, measured at 550 - 620nm, is directly proportional to the number of living cells in the culture. Reagents in the kit have been carefully formulated and optimised for sensitivity, assay robustness and automation.

• Safe – Non-toxic

• Sensitive and accurate – as low as 950 cells can be accurately quantified

• Fast – high-throughput assays readily automated with HTS liquid handling systems

• Homogeneous and convenient – a single reagent; mix, incubate and measure

Applications

• Cell Proliferation assays: effects of cytokines, growth factor and nutrients

• Cytotoxicity and Apoptosis assays: evaluation of toxic compounds, anti-cancer antibodies and toxins

• Drug Discovery: high-throughput screening for anti-cancer drugs.

Storage conditions

Store the reagent at -20°C. Keep Assay Buffer and Solubilisation.
Solution at 4°C and room temperature, respectively.
Shelf life: 12 months.

     QMTT Cell Viability Assay Kit Manual

     QMTT Cell Viability Assay Kit MSDS

    QMTT Cell Viability Assay Kit Certificate of Analysis
 

 WST-1 Cell Proliferation Assay Kit

Description

WST-1 Cell Proliferation Assay Kit provides a safe and simple method to measure cell proliferation and cell viability. This colourimetric assay is based on the reduction of tetrazolium (WST-1) salts to coloured formazan compounds by living (metabolically active) cells. Tetrazolium salt (WST-1) is cleaved to soluble Formazan dye by mitochondria dehydrogenase, which is only active in viable cells. The increase of enzyme activity leads to an increase of the production of Formazan dye, so the quantity of Formazan dye measured by 450nm absorbance is directly proportional to the living cells in culture. The procedure is rapid, accurate and can assay as low as 1000 cells/well
(assay range is 1000 to 100,000 cells /well).

• Easy and Simple – add one solution to the cells, incubate and read absorbance

• Fast – short reaction time, no centrifuge needed

• Flexible – plates can be read and re-incubated (if required) for further colour development

• Accurate – absorbance directly correlates to number of living cells

• Sensitive – more sensitive than MTT, MTS, XTT-based assays

• Reliable – high reproducibility of data

Effect of cell number on absorbance at 450nm measured using WST-1 Cell Proliferation Assay Kit Various numbers of K562 cells were added to the wells of a 96-well plate in 100ml of DMEM supplemented with 10% FBS. The medium was allowed to equilibrate at 37°C, 5%CO2 for 30 min. 10 ml of 1:1 mixture of WST-1 solution and Electron Coupling Solution was added to the wells and allowed to incubate for 2 hrs at 37°C, 5% CO2 atmosphere. The absorbance at 450nm was measured using an ELISA plate reader (reference wavelength 620nm). Each point represents the mean ± SD of 4 replicates. The correlation coefficient of the line was 0.996.

     WST-1 Cell Proliferation Assay Kit Manual

     WST-1 Cell Proliferation Assay Kit MSDS

     WST-1 Cell Proliferation Assay Kit Certificate of Analysis
 

 Malachite Green Phosphate Assay Kit

Description

The Malachite Green Phosphate Assay Kit is based on quantification of the green complex formed between Malachite
Green, molybdate and free orthophosphate. The rapid colour formation from the reaction can be conveniently measured on a spectrophotometer (600 - 660nm) or on a plate reader. The assay is simple and fast, involving a single addition step for phosphate determination.

• Reagent very stable – no filtration of reagent required

• High sensitivity and wide detection range – detect from 1.6pmoles of phosphate (useful range between 0.02µM and 20µM phosphate)

• Fast and convenient – homogeneous “mix-andmeasure assay completed within 10 minutes

• Compatible with routine laboratory and HTS formats - assays performed in tubes, cuvettes or microplates

• Applications - phosphatase assays, lipase assays, nucleoside triphosphatase assays, high-throughput screen for phosphatase inhibitors

Malachite Green Phosphate Assay, kinetics of the chromogenic reaction.

80µL of standard phosphate solution was transferred to a clear-bottom 96-well plate. 20µL of the Malachite Green Reagent was added and OD at 650 nm was read every minute. The chromogenic reaction was completed within 10 minutes. The signal was stable between 10 and 20 minutes.

Storage conditions

The Malachite Green Reagent and standard is stable for one year when stored at 4°C.



 

     Malachite Green Phosphate Assay Kit Manual

     Malachite Green Phosphate Assay Kit MSDS

     Malachite Green Phosphate Assay Kit Certificate of Analysis