Exact Polymarase
 
For High-Fidelity Hot-Start PCR
 
 The Exact Polymerase ensures a robust PCR performance in high-fidelity PCR and gives trouble-free and highly specific amplification. The combination of Exact Polymerase, a unique hot-start high-fidelity PCR enzyme, and the Exact PCR Buffer minimizes the need for PCR optimization and maintains an optimum of high fidelity and PCR product yield.
Owing to its exceptionally high proofreading activity, this enzyme generates PCR products with more than 10 times the accuracy of Taq DNA polymerase, providing the ideal tool for all PCR applications that require a low error rate, such as cloning and site-directed mutagenesis. The Enzyme fidelity varies from 2.3 x 10–6 to 3.6 x 10–6 misincorporated bases per PCR product doubling . These values are more than 10 times better than Taq DNA polymerase, as determined by several measurements and are comparable to or better than those of other proofreading enzymes.
 
PCR was carried out using Exact Polymerase (5 PRIME), proofreading polymerases from the indicated suppliers, or a commercial Taq/proofreading polymerase mixture from Supplier R (R’). Parallel reactions were performed following suppliers’ recommendations, using 200 ng HeLa genomic DNA. A 1.5 kb fragment of the human hypoxanthine ribosyl transferase gene was amplified in 35 PCR cycles.
M: markers.
 
 

Features
 

  • Robust amplification with Hit-Start Enzyme

  • Accurate products be efficient proofreading activity

  • Minimal PCR optimisation due to special buffer formulation

Applications
 

  • Cloning

  • Site-directed mutagenesis

  • Mutation analysis

PCR Enzyme Selection Guide
 

Product/
Application

PCR

 RT-PCR Real-Time Quantitive PCR
Routine PCR Complex Targets High Fidelity/Low Error Rate PCR GC-Rich Targets Long Range PCR up to 40kb One Step RT-PCR Two Step RT-PCR SYBR Green Detection Probe Based Detection
Taq DNA Polymerase *** ** - - - - ** - -

Master Taq
 

 *** ** - *** *** - ** - -
PCR Extender System * ** ** ** *** - ** - -
5 PRIME MasterMix *** ** - * - - ** - -
HotMaster Taq DNA Polymerase *** *** - - - - *** - -
5 PRIME HotMasterMix *** *** - - - - ***    
Masterscript Kit - - - - - - ***2 ***1 ***1
Masterscript RT-PCR System - - ***3 - - *** *** - -
RealMasterMix - - - - - - ***1 *** ***4
RealMasterMix Probe - - - - - - ***1 - ***
Exact Polymerase * ** *** * - * - - -
 

Key. ***. Perfect ***. Good *. Acceptable -. Not Recommended

1-For Two-Step RT-PCR 2-Can be used with any other PCR kit for the PCR part 3- For RT-PCR 4-For single-plexassays

 
Order CodeDescription

Size

Price

FPR0600Exact Polymerase - 100 U

PK1

£69.00

FPR0610Exact Polymerase - 500 U

PK1

£276.00

  

Tel: +44 (0) 115 977 5494 - Fax: +44 (0) 115 945 5379    Email us
Copyright Flowgen Bioscience Limited © 2006 - 2008. All rights reserved
Company Registered in England & Wales - Registration No.: 05248645 - VAT Registration No.: GB848199573