Genomic DNA Extraction Kit (Blood/Bacteria/Cultured Cells)

Genomic DNA MIni Kit
Sample: 300µl Whole Blood, 107 Cultured Cells,
109 Bacteria (Gram +/-), 107 Yeast
Average Yields: 200µl Whole Blood -6µg, 200µl
Buffy-50µg, 5 x 106Lymph./Cult. Cells -50µg

Genomic DNA Maxi Kits
Sample (Protocols Included): Fresh/ Frozen Blood
Average Yields: 140/75µg

Description

Genomic DNA Extraction Kits (Blood/Bacteria/Cultured Cells) provides a fast and economical method for purification of total DNA (including genomic, mitochondrial and viral DNA) from whole blood (fresh/frozen), plasma, serum, buffy coat, other body fluids, lymphocytes and cultured cells. This kit is also ideal for bacterial and cultured cells. The blood protocol utilises RBC lysis buffer, GB buffer and a rapid heating step to release DNA into solution. DNA in the chaotropic salt solution binds to the glass fiber matrix of column. After washing off the contaminants, the purified DNA is eluted by low salt elution buffer or water. There is no requirement for phenol/chloroform extraction or alcohol precipitation. Purified DNA of approximately 20-30 kb is suitable for PCR or other enzymatic reactions.

Applications
-PCR
-Southern blotting
-PADP/ AFLP

Product Intended Use (Research/Clinical Application)*
 

Product Information
 
FYGB50
50 mini preps/kit
50 mini preps / kit
RBC Lysis Buffer: 60ml
GB Buffer: 15ml
GT Buffer: 15ml
W1 Buffer : 25ml
Wash Buffer (Concentrated): 12.5ml
Elution Buffer : 30 ml
Proteinase K : 11 mg
GD Columns: 50 pcs
2 ml Collection Tubes: 100 pcs

FYGB100
100 mini preps/kit
100 mini preps / kit
RBC Lysis Buffer: 120ml
GB Buffer: 30ml
GT Buffer: 30ml
W1 Buffer : 45ml
Wash Buffer (Concentrated): 25ml
Elution Buffer : 30ml
Proteinase K : 11 mg x 2
GD Columns: 100 pcs
2 ml Collection Tubes: 200 pcs

FYGBM10
10 maxi preps/kit
10 maxi preps / kit
RBC Lysis Buffer: 125ml & 250ml
GB Buffer: 120ml
W1 Buffer : 45ml
Wash Buffer (Concentrated): 50 ml
Elution Buffer : 60 ml
50ml Centrifuge Tube: 10 pcs
GD Maxi Columns: 10 pcs

FYGBM25
25 Maxi preps/kit
25 maxi preps / kit
RBC Lysis Buffer: 500ml x 2
GB Buffer: 265ml
W1 Buffer : 100ml
Wash Buffer (Concentrated): 50ml x 2
Elution Buffer : 125ml
50ml Centrifuge Tube: 25 pcs
GD Maxi Columns: 25 pcs
 

 Genomic DNA Extraction Kit (Tissue)

Sample: 20mg of tissue,0.5 cm of mouse tail
Protocols Incl.: Tissue, Paraffin-embedded tissue,Buccal Swab
Yield: up to 50μg of DNA
Operation time: 40-60 mins
 

Description

Genomic DNA Extraction Kit (Tissue) has been designed for purification of total DNA (including genomic, mitochondrial and viral DNA) from a variety of animal tissues or cells. The provided micropestle can efficiently homogenize tissue sample to shorten the time spent for lysis. The method uses proteinase K and a chaotropic salt, guanidine hydrochloride to lyses cells and degrade protein. DNA in chaotropic salt binds to the glass fiber matrix of column. After washing off the contaminants, the purified DNA is eluted by low salt elution buffer or water. Purified DNA of approximately 20-30 kb in length is suitable for PCR or other enzymatic reactions.

Applications
-PCR
-Southern blotting
-PADP/ AFLP

Quality Control
The quality of Genomic DNA Extraction Mini Kit (Tissue) is tested on a lot-to-lot basis. The kits are tested by isolation of genomic DNA from 10mg of mouse liver. Purified DNA is quantified with a spectrophotometer. Genomic DNA yield is more than 10μg with A260/A280 ratio 1.7 to 1.9 .

Product Information

FYGT50
50 Mini preps/kit
50 mini preps / kit
GT Buffer: 15ml
GB Buffer: 15ml
W1 Buffer: 25ml
Wash Buffer (Concentrated): 12.5ml
Elution Buffer : 30 ml
Proteinase K: 11mg
GD Columns: 50 pcs
2 ml Collection: 100 pcs
Tubes Micropestles: 50 pcs

FYGT100
100 Mini preps/kit
100 mini preps / kit
GT Buffer: 30ml
GB Buffer: 30ml
W1 Buffer : 45ml
Wash Buffer (Concentrated): 25ml
Elution Buffer : 30ml
Proteinase K: 11mg x 2
GD Columns: 100 pcs
2 ml Collection: 200 pcs
Tubes Micropestles: 100 pcs