Protein A SuperSpin

Protein A SuperSpin is supplied for fast small-scale purification of immunoglobulin G (IgG) from various species (e.g. serum, ascites and mammalian cell culture broth etc). Protein A has been well proven a powerful affinity ligand which shows very high specificity to IgGs from mammalian hosts.

Each Protein A SuperSpin tube accommodates 50µl of Protein A agarose particles. The affinity resin is specifically designed to provide large pore size with small particle size (i.e. 20 – 50µm). So large antibody molecules can access the affinity ligand rapidly with excellent mass transfer rate. As a result, half milligram quantity of antibody can be easily purified within 20 minutes with inexpensive cost.  No special expensive chromatography instrument and column is required.

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Protein A SuperSpin is particularly useful for the following applications:

• The quantity of antibody to be purified is small (less than half milligram)

• On-line detection of antibody expression level

• High throughput purification of antibodies

• High throughput screening of antibodies

Key benefits:

• Rapid antibody capture and recovery

• High antibody binding capacity (half milligram)

• High throughput format (24 samples can be processed in parallel)

• Low cost

• Easy to use and no expensive equipment required